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on a heavy glass plate raised to the temperature of the. A high proportion of platelets are transfused prophylactically to reduce the risk of spontaneous bleeding in patients who are thrombocytopenic after chemotherapy or hematopoietic progenitor cell transplantation. AABB recently published updated guidelines for platelet transfusion . Because of the increased risk of hemorrhage, the National Comprehensive Cancer Network . Prophylactic platelet transfusions are not recommended for patients with normal platelet counts who are undergoing cardiac surgery with cardiopulmonary bypass. Transfusion should be reserved for patients experiencing perioperative bleeding with evidence of thrombocytopenia or platelet dysfunction. Antiplatelet medications are prescribed to prevent thrombosis in patients with coronary artery disease, stroke, transient ischemic attacks and peripheral arterial disease. When patients taking these medications develop bleeding or require an emergent invasive procedure, the antiplatelet effect needs to be rapidly reversed. Specific antidotes are not available. Randomized clinical trials have not been conducted and there are no evidence based guidelines. Five observational studies have not shown any benefit for transfusion of platelets receiving antiplatelet agents who present with traumatic brain injury. The recent transfusion literature contains some recommendations for platelet transfusion therapy in these situations. The standard dose of aspirin varies from 81 to 325 mg per day. Aspirin is immediately absorbed after ingestion and irreversibly inhibits platelets within 15 to 30 minutes by binding to cyclooxygenase 1 . The half life of aspirin is 30 minutes. Aspirin has a low bleeding risk. Discontinuation of aspirin for 2 days will usually result in production of enough new platelets to provide adequate hemostasis for elective surgery. Aspirin effect on platelets can be assessed by platelet function testing on PFA-100. Aspirin effect includes a prolonged closure time with epinephrine and a normal closure time with ADP. Dipyridamole inhibits 2,3 phosphodiesterase enzyme in platelets and inhibits ADP induced platelet aggregation. By itself, dipyridamole is considered to be a weak antiplatelet medication that seldom causes bleeding. Aggrenox is a more effective antiplatelet medication that is often prescribed for patients with a history of stroke. It is a combination of 25 mg aspirin and 200 mg of dipyridamole. Bleeding risk is low. Platelet transfusion is usually not necessary. The effect of dipyridamole can only be assessed by whole blood platelet aggregation. Clopidogrel inhibits platelet function within 2 to 4 hours, while a daily dose of 75 mg becomes effective within 24 hours and has maximum effect within 4 to 7 days. Circulating half life is 7 to 8 days. Daily clopidogrel increases the risk of bleeding. Clopidogrel should be discontinued before elective surgery. At least 3 clinical practice guidelines provide direction regarding the timing of surgery in patients receiving Plavix. Each of these guidelines recommends discontinuing Plavix for at least 3 to 7 days prior to surgery to allow platelet function to return to normal and decrease the risk of bleeding. The PATCH trial was a multicenter randomized trial that investigated the effectiveness of platelet transfusion for patients on antiplatelet medications who developed intracranial hemorrhage . Antiplatelet medications included aspirin alone, aspirin plus dipyridamole, clopidogrel alone and aspirin plus clopidogrel. Patients transfused with platelets had more adverse events during their hospital stay and a higher mortality rate. Platelet transfusion seemed inferior to standard care for patients who develop intracranial hemorrhage while taking antiplatelet therapy. Platelet transfusion is not recommended for these patients . Like clopidogrel, prasugrel is also irreversibly inhibits binding of ADP to the platelet P2Y12 receptor. It is a prodrug which is metabolized to its active form by the cytochrome P450 enzyme pathway. Prasugrel is given as a loading dose of 60 mg followed by daily doses of 10 mg for patients weighing more than 60 kg. The daily dose is reduced to 5 mg per day for patients weighing less. Onset of action and circulating half life are similar to clopidogrel. Prasugrel has a higher bleeding risk than clopidogrel. Prasugrel should be discontinued for 5 to 7 days before elective surgery. Platelet function can be assessed with the VerifyNow assay. One dose of apheresis platelets may be needed for bleeding or urgent surgery. Selective serotonin reuptake inhibitors, such as fluoxetine, sertraline, paroxetine, and clomipramine, decrease the uptake of serotonin by platelets. Since platelets are unable to synthesize serotonin, these medications lower the intracellular serotonin concentration, inhibit platelet aggregation and increase the risk of abnormal bleeding including menorrhagia, metorrhagia, upper GI hemorrhage, cerebral hemorrhage, hematuria, hemoptysis, hemarthrosis, and post-op bleeding. Platelet transfusions will probably not be effective in controlling bleeding until after these medications have been discontinued for several half-lives. Cryoprecipitate transiently corrects uremic platelet defects. Reversal of a qualitative platelet defect requires 10 units of cryoprecipitate.Improvement in platelet function may not be evident for up to 4 hours and lasts approximately 24 hours. Repeat doses of cryoprecipitate are less beneficial. Dialysis also corrects the uremic qualitative platelet defect. Thus, the use of cryoprecipitate to correct a qualitative platelet defect should be reserved for life-threatening hemorrhage or prior to an invasive procedure. Platelet transfusions for patients with platelet consumptive disorders . Goel R, Ness PM, Takemoto CM, Krishnamurti L, King KE, Tobian AA. Platelet transfusions in platelet consumptive disorders are associated with arterial thrombosis and in-hospital mortality. Blood. 2015 Jan 14. pii: blood-2014-10-605493.. are

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